Fig 1: The GRHL2 direct and indirect targets EpCAM, RAB25, and CD133 are differentially expressed in each CTC line. (A) Comparison of EPCAM expression in CTC lines and SW620 cells (microarray data). (B) Relative EPCAM gene expression in each CTC line and in SW620 cells. (C) EpCAM protein expression in the nine CTC lines by immunofluorescence analysis. (D) Comparison of RAB25 expression in CTC lines and SW620 cells (microarray data). (E) RAB25 gene expression profile in each CTC line and in SW620 cells. (F) Immunofluorescence staining of RAB25 protein in the nine CTC cell lines. (G) Comparison of CD133 in CTC lines and SW620 cells (microarray data). (H) CD133 expression pattern in CTC lines and SW620 cells. (I) CD133 protein detection by immunofluorescence analysis. The scale bars denote 20 μm. h: p-value = 1.57 × 10−23; i: p-value = 1.34 × 10−18. All the RT-qPCR results were normalized to the B2M expression level in each sample; * p < 0.05, ** p < 0.01, *** p < 0.001 compared with SW620 cells.